Abstract
Bacopa monnieri is one of the most interesting plants from the Ayurveda system. The aims of present research were, basing on in vitro shoot culture of B. monnieri, to determine content and to evaluate the influence of physiologically important metabolites on the selected bioelements accumulation in biomass. The most significant increase in biomass production was observed in the culture medium enriched with 0.5 mg/L of anthranilic acid. In this medium also, the highest accumulation of Mg was noted. The highest concentration of iron was determined in B. monnieri in vitro culture enriched with 0.25 g/L of serine. The addition of l-tryptophan, magnesium sulfate, and zinc hydroaspartate caused only a small increase in the accumulation of copper in B. monnieri. Increase in Zn accumulation was obtained in biomass from in vitro culture of B. monnieri with the addition of magnesium sulfate and zinc hydroaspartate. In the case of Na, the maximum level of this element was in biomass from medium enriched with zinc hydroaspartate. Twofold increase in K concentration was obtained in biomass from cultures on medium with addition of serine and magnesium sulfate. The concentrations of Ca in biomass of all studied media were at the similar level.
Highlights
Bacopa monnieri (L.) Pennell, known locally in India as Brahmi or Jalanimba, is one of the most important plants in the traditional Hindu system ofM
The lower biomass increments were obtained on the medium containing 0.1 g/L L-tryptophan (9-fold) and the lowest in the case of the medium with addition of 0.1 g/L Zn (8-fold)
The obtained results illustrate the influence of chosen organic compounds on accumulation of bioelements in in vitro shoot cultures of B. monnieri
Summary
The aim of the current work was to create composition of medium which guarantees the optimal increase of biomass, optimal concentration of chosen organic compounds which lead to maximal accumulation of the selected physiologically active elements
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