Abstract
AbstractAnthurium andraeanumis one of the most economically important floral crops and potted flowers marketed worldwide. Microsatellite markers are currently the preferred molecular marker owing to the many desirable attributes, including hypervariability, codominance, and amenability to high-throughput genotyping; however, there are few polymorphic molecular markers available for Anthurium. The object of this study was to develop and characterize novel microsatellite markers using the Araceae sequences in GenBank of the National Center for Biotechnology Information (NCBI) to contribute to molecular identification for cultivar protection. Using 1,579 Araceae expressed sequence tags (ESTs) and the related nucleotide sequences, 100 candidates contained simple sequence repeat (SSR) motifs that were suitable for primer design. Furthermore, 100 pairs of SSR primers were screened against a set of 28 diverse genotypes representing 24 cultivars that included four registration cultivars which were bred from the Taiwan Agricultural Research Institute (TARI) and 20 commercial cultivars, appended with three hybrid progeny and a mutant line. From the selected six polymorphic SSR loci, 52 alleles were amplified and 27 distinct genotypes were found, except for ‘Tropical’ and its mutant, with a mean number of eight alleles per locus. The polymorphism information content (PIC) ranged from 0.86 to 0.93. Based on these results, we proposed a key identification set using four microsatellite markers that is sufficient to discriminate among 24 cultivars. Because the Anthurium microsatellite markers developed in this study are primarily from expressed sequence tags or related genomic sequences, they can be used for cultivar identification and, accordingly, contribute to genetic evaluations in breeding programs.
Highlights
Anthurium andraeanum is one of the most economically important floral crops and potted flowers marketed worldwide
A number of genomic markers based on DNA polymorphism, such as random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) molecular markers, have been developed and used for genetic diversity analyses (Wang et al 2001; Nowbuth et al 2005)
A total of eight (14.5 %) SSR-containing primers were designed from 55 nucleotide sequences of Anthurium
Summary
Anthurium andraeanum is one of the most economically important floral crops and potted flowers marketed worldwide. The present study aims at mining the genomic information of Anthurium and other Araceae genera for developing novel and specific SSR markers and to select critical markers for fingerprint analyses in the future For this purpose, we screened the NCBI GenBank of publicly available nucleotide sequences and expressed sequence tags (ESTs) for the SSR motifs, designed flanking primer pairs to amplify the SSR-containing markers, evaluated a key subset of polymorphic primers for the identification of cultivars, and tested the marker transferability across the Araceae family
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