Abstract
Background: Cholesterol estimation in eggs was mostly done by High performance liquid chromatography (HPLC) and gas chromatography which are tedious, expensive and time consuming methods. Thus, there is a need to determine cholesterol content by an easy spectrophotometric method which is not expensive and tedious. The present study was aimed to determine the cholesterol content in chicken eggs spectrophotometrically so as to provide better information about the nutritional quality of an egg. Methods: The experiment was conducted on 24 healthy layer birds of Dahlem Red (DR). 45 egg samples were randomly collected fresh in the morning. Total cholesterol was determined by enzymatic colorimetric method by using biochemical estimation kit (Agappe Diagnostics Ltd.). The pure yolk was separated and determined enzymatically for cholesterol estimation. The method is newly evaluated, rapid, simple and accurate. Result: The methodology designed herein can provide specific, accurate and suitable method for estimation of total cholesterol in chicken eggs. The average cholesterol content of Dahlem red was 12.59±0.09 mg/ml in egg yolk or 195.66±2.80 mg/egg yolk.
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