Development of Novel Markers and Creation of Non-Anthocyanin and Anthocyanin-Rich Broccoli (Brassica oleracea var. italica) Cultivars

Abstract

In broccoli, anthocyanin pigments can be accumulated in the flower bud epidermis, resulting in a purple-green head. This study aimed to create non-anthocyanin green broccoli varieties and anthocyanin-rich purple broccoli varieties using new F3′H and Pur7.1-K1 molecular markers, respectively. The breeding program started with crosses of the recipient (superior variety and line) LF02 line with the donor line SN60 carrying the recessive allele f3′h and the donor line BT126 carrying the dominant allele Pur7.1. The F1 hybrids were confirmed with molecular markers and backcrossed with the recurrent parent LF02, followed by cycles of foreground and background selection at each stage. A total of 161 green plants with the f3′hf3′h genotype and 152 purple plants with the Pur7.1Pur7.1 genotype were selected from the BC3F2 line. Among these, 34 green plants and 28 purple plants demonstrated >85% background recovery. The identified plants were selfed to obtain 301 green and 416 purple BC3F3 plants for assessment of major agronomic traits. After these investigations, two green broccoli lines without anthocyanin and three anthocyanin-rich purple lines with the best yield/quality characteristics were obtained. The development of these lines might help provide basic materials and the theoretical basis for breeding commercial broccoli varieties.