Abstract
In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture of Ab155 antibody (with fixed concentration) and sulfapyridine. In order to obtain a highly sensitive biosensor, Ab155 was immobilized onto magnetic latex nanoparticles surface to create a 3D architecture (Ab-MLNp). Using electrochemical impedance spectroscopy (EIS), we investigated the influence of the Ab-MLNp on the sensitivity of our approach. The optimized system was analyzed, as competitive assay, with different concentrations of sulfapyridine (40 µM, 4 µM, and 2 nM) and with phosphate buffer solution. From data fitting calculations and graphs, it was observed that the EIS showed more linearity when Ab-MLNp was used. This result indicates that the magnetic latex nanoparticles increased the sensitivity of the biosensor.
Highlights
Increasing attention has been paid to antibiotics as aquatic micropollutants with their environmental fate and impact to be understood [1]
The immunoreagents for sulfapyridine (SPy) detection used for the development of the biosensor were described before [23]
The characterization of the produced nanoparticles was performed by transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential measurement, and thermal gravimetric analysis (TGA)
Summary
Increasing attention has been paid to antibiotics as aquatic micropollutants with their environmental fate and impact to be understood [1]. Among the SAs, sulfapyridine, which is commonly used in aquaculture, was frequently detected in various environmental waters (e.g., wastewater effluents and receiving water bodies as well as fish farms and adjacent water bodies) [5]. For the detection of sulfapyridine, various methods have been used, such as chromatographic methods (likely high-performance liquid chromatography coupled with mass spectrometric detection (HPLC-MS)). Such methods have been applied due to their sensitivity and compound quantification data. The above-mentioned approaches are time consuming and require complex sample preparation procedures, expensive laboratory equipment, and skilled professionals to handle these techniques
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