Development of novel inorganic strontium nanoparticles for delivery of pDNA and siRNA to breast cancer cell

Abstract

Event Abstract Back to Event Development of novel inorganic strontium nanoparticles for delivery of pDNA and siRNA to breast cancer cell ATHIRAH BAKHTIAR1*, Md Ezharul H. Chowdhury2, Iekhsan Othman3 and Anuar Zaini3 1 Faculty of Pharmacy, Mahsa University, Malaysia 2 Monash University Malaysia, Malaysia 3 Monash University, Australia Background Recent studies have been focused on developing smart nanoparticles for efficient delivery of transgenes and siRNAs into cancerous cells of an animal model through active and passive targeting. Precipitation reaction is one of the facile and convenient ways to generate inorganic strontium nanoparticles, in which an insoluble salt is formed after mixing two water-soluble salts. Advantages in comparison with other methods include requiring only simple equipment, ability to prepare and control particle size and composition in near ambient temperature and pressure. The approach would pave a new way for facile, low toxicity and large-scale synthesis of inorganic nanoparticles with well-controlled dimensions and properties. Methods The study involved the formation of 5 different types of inorganic strontium nanocrystals via precipitation reactions, with various observational studies involving pH, temperature, salt concentration and time of incubation. Strontium particle sizes were determined using zetasizer and scanning electron microscope prior to binding studies. The nanoparticles were then subjected to various cellular studies including cellular uptake and cytotoxicity studies through incorporation of p53 and MAPK siRNA, forming gene-nanoparticle complexes onto human and mice mammary carcinoma cells, MCF-7 and 4T1 cells, respectively. Unloaded strontium nanoparticles formed were also observed for toxicity effect against both cells. Results Studies showed that strontium fluoride and strontium carbonate were smaller in size in comparison to other strontium salt particles, averaging in 100-300nm in diameter. Additionally, cellular studies revealed that gene-loaded strontium sulfite and strontium fluoride were responsible for greater cytotoxicity effect, in comparison naked gene treatment. Low toxicity effect was seen with unloaded strontium nanoparticles. Studies were compared against established inorganic carbonate apatite nanoparticles. Conclusion Nano-sized strontium particles showed great potential to be applied for in vivo studies, ideally seen with strontium sulfite and strontium fluoride, via the gene delivery system in the cellular level. Keywords: breast cancer, pDNA delivery, siRNA, Strontium, Nanoparticles Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Cancer Citation: BAKHTIAR A, Chowdhury MH, Othman I and Zaini A (2019). Development of novel inorganic strontium nanoparticles for delivery of pDNA and siRNA to breast cancer cell. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00137 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. ATHIRAH BAKHTIAR, Faculty of Pharmacy, Mahsa University, Bandar Saujana Putra, Selangor, 42610, Malaysia, athirahbakhtiar@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers ATHIRAH BAKHTIAR Md Ezharul H Chowdhury Iekhsan Othman Anuar Zaini Google ATHIRAH BAKHTIAR Md Ezharul H Chowdhury Iekhsan Othman Anuar Zaini Google Scholar ATHIRAH BAKHTIAR Md Ezharul H Chowdhury Iekhsan Othman Anuar Zaini PubMed ATHIRAH BAKHTIAR Md Ezharul H Chowdhury Iekhsan Othman Anuar Zaini Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.