Abstract
In vitro fertilization of mammalian ova has been successfully carried out in the rabbit1–3 and the hamster4,5, but development of fertilized ova beyond the two-cell stage has not been achieved6. Brinster et al.7 have observed fertilization and blastocyst development in explanted Fallopian tubes using organ culture. More recently, Whittingham8 has successfully fertilized mouse eggs in vitro, transplantation of which into pseudo-pregnant recipient mothers yielded 17 day old foetuses. The present studies confirm the results of Whittingham8 and further demonstrate that apparently normal full-term progeny can be obtained from implantation of in vitro fertilized eggs that have been maintained in culture to the blastocyst stage.
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