Development of Ni(II) resistant S. cerevisiae and its application: Adsorption study and modeling

Abstract

The study aims to develop Ni(II) resistant Saccharomyces cerevisiae to decontaminate high Ni(II) concentrations from an aqueous system. Initially, two different microorganisms were taken: Bacillus circulans MTCC 3161, Saccharomyces cerevisiae. For these two strains, the experiments were carried out for successive screening for survival/tolerance, minimum inhibitory concentration (MIC), and biosorption capacity for Ni(II) from an aqueous solution. Ni(II) resistant Saccharomyces cerevisiae AJ208 showed a MIC of 5500 mg/L for Ni(II). Nucleotide sequences of Saccharomyces cerevisiae AJ208 were deposited in the Gene bank. All experiments were conducted to determine the effects of various physical conditions, such as pH, age and volume of inoculum, temperature, and incubation time, the volume of fermentation medium. The characterization of the Saccharomyces cerevisiae AJ208 was carried out using SEM-EDAX, FTIR. The Langmuir isotherm and pseudo-second-order kinetic models are well fitted with the experimental data. The Langmuir maximum adsorption capacity is 170.06 mg/g. The thermodynamic studies showed the mechanism of Ni(II) removal is an endothermic and spontaneous reaction. The experimental data have been analyzed using statistical method (MLR) and Genetic algorithm (GA). This study reports the highest Ni(II) resistant Saccharomyces cerevisiae AJ208 (5000 mg/L) and also the feasibility of Ni(II) removal from 3000 mg/L initial Ni(II) concentration into an aqueous solution, which could be of great interest as a potential reference strain for Ni(II) removal.