Abstract
We used double digest restriction site associated DNA sequencing (ddRAD) to develop new geographically informative nuclear SNP loci in Quercus robur and Quercus petraea. Genotypes derived from sequence data of 95 individuals covering the distribution range of the species were analysed to select geographically informative and polymorphic loci within Russia and Germany. We successfully screened a selected set of 119 loci on a MassARRAY® iPLEX™ platform on 190 individuals from 19 locations in Russia. The newly developed loci will be useful for genetic studies over the whole distribution range of both species.
Highlights
Keywords Quercus robur · Quercus petraea · Single nucleotide polymorphism · MassARRAY
For SNP discovery, we used leaf or cambium material from 95 Q. robur and Q. petraea trees originating from all Europe, Ukraine and Russia (Table 1)
A final selection of 168 loci was used to design four MassARRAY ® iPLEXTM multiplexes (Assay Design Suite v2.0 [Agena BioscienceTM, San Diego, USA]), which included a total of 130 loci (Supplementary Material S1)
Summary
Keywords Quercus robur · Quercus petraea · Single nucleotide polymorphism · MassARRAY Quercus robur (L.) and Quercus petraea (Matt.) are very common tree species in many forests in Europe. The distribution edge of Quercus robur goes a few hundred kilometres more to the East up to the South-Ural region in Russia.
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