Abstract
Abstract Microsatellite markers have been used extensively in studies of sperm competition and the genetic diversity of organisms. In the present study, we performed transcriptome sequencing for Velarifictorus aspersus and developed simple sequence repeat markers to identify valuable markers for genetic studies. The average total length of the V. aspersus transcriptome sequence was 7.70 Gb and it contained 7878 microsatellite loci. Sixty-four microsatellite loci were randomly selected for primer design and 39 loci were successfully amplified, among which 23 loci were polymorphic. The microsatellites were also tested in six other species from the Gryllidae family. Among the species belonging to the genus Velarifictorus, 22 loci were amplified in Velarifictorus micado, eight loci in Velarifictorus ornatus, and 3–6 loci in the other four cricket species.
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