Development of new methods for subcellular diagnostics and therapy of atherosclerosis aimed at eliminating dysfunctional mitochondria.

Abstract

Background and Aims: Our goal was investigated the relationship of PpIX-induced 5-ALA accumulation on platelet-derived cybrid lines with mutated mitochondria and THP-1 monocytes. There is a photosensitizer protoporphyrin IX (PpIX) widely used in clinical practice, which is produced by 5-aminolevulinic acid (5-ALA) in mitochondria in a cascade of heme synthesis reactions. When 5-ALA is supplied from the outside, the overproduced PpIX cannot be quickly utilized by the available amount of ferrochelatase into the final product - heme and, therefore, accumulates in cells at an increased concentration.