Abstract
The fabrication of collagen-based biomaterials for skin regeneration offers various challenges for tissue engineers. The purpose of this study was to obtain a novel series of composite biomaterials based on collagen and several types of clays. In order to investigate the influence of clay type on drug release behavior, the obtained collagen-based composite materials were further loaded with gentamicin. Physiochemical and biological analyses were performed to analyze the obtained nanocomposite materials after nanoclay embedding. Infrared spectra confirmed the inclusion of clay in the collagen polymeric matrix without any denaturation of triple helical conformation. All the composite samples revealed a slight change in the 2-theta values pointing toward a homogenous distribution of clay layers inside the collagen matrix with the obtaining of mainly intercalated collagen-clay structures, according X-ray diffraction analyses. The porosity of collagen/clay composite biomaterials varied depending on clay nanoparticles sort. Thermo-mechanical analyses indicated enhanced thermal and mechanical features for collagen composites as compared with neat type II collagen matrix. Biodegradation findings were supported by swelling studies, which indicated a more crosslinked structure due additional H bonding brought on by nanoclays. The biology tests demonstrated the influence of clay type on cellular viability but also on the antimicrobial behavior of composite scaffolds. All nanocomposite samples presented a delayed gentamicin release when compared with the collagen-gentamicin sample. The obtained results highlighted the importance of clay type selection as this affects the performances of the collagen-based composites as promising biomaterials for future applications in the biomedical field.
Highlights
This article is an open access articleThe design of collagen biomaterials for use as implants poses various challenges for tissue engineers [1]
30B) in the synthesis and characterization of type II collagen-based biomaterial with collagen are available, none of them provides a systematic comparison between the our study offers valuable information about the importance of clay type sele uses of Cloisite clay type series
Fourier-Transform Infrared Spectroscopy (FTIR) spectra confirmed the inclusion of clay in the collagen polymeric matrix without positioned at 1240 cm−1 is assigned to the NH bending vibrations from amide linkag any denaturation of triple helical conformation where the specific peaks of clay corre[32]
Summary
This article is an open access articleThe design of collagen biomaterials for use as implants poses various challenges for tissue engineers [1]. Hydrogels are 3D crosslinked polymers structures with high capacity to absorb and retain important water amounts without the degradation of their tridimensional network. Hydrogels can serve as matrices for the obtaining of composite biomaterials, targeting adequate structures and morphologies beneficial for biomedical application [7,8,9,10]. Because of their availability and versatility, natural polymers such as collagen, alginate, chitosan, hyaluronic acid, and cellulose are mostly preferred for their use in various formulations and mixtures
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