Development of new chemiluminescence biosensors for determination of biogenic amines in meat

Abstract

Development of chemiluminescence one-shot biosensors for determination of biogenic amines is described and compared with high-performance liquid chromatography (HPLC) method coupled with pre-column derivatisation.The biosensors are based on enzymatic oxidation to 4-aminobutyraldehyde with putrescine oxidase or diamine oxidase as catalysts. The lowest measured concentration for the biosensor with putrescine oxidase was 1mg/L. The detection limit, calculated as 3σ value, was 0.8mg/L. The biosensor with diamine oxidase had the lowest measured concentration of 1mg/L of putrescine. Detection limit, calculated as 3σ value, was 1.3mg/L.Biosensors were tested on five different meat samples, and the results were compared with HPLC coupled with pre-column derivatization.Results showed that new biosensors could be used in determination of putrescine concentration in meat samples but improvements, such as sample pretreatment before determination or design of interference free biosensor, are required.