Development of nanoliposome formulation of beta-carotene using high speed homogeniser method

Abstract

Background: Beta carotene has hydrophobic and unstable properties. Nanoliposomes can package various types of drugs, including hydrophilic and hydrophobic drugs, as well as biological substances such as DNA, RNA, and proteins. This allows for more efficient and targeted drug delivery, making it possible to formulate beta-carotene nanoliposomes to improve efficacy and stability. Objective: To formulate and characterise betacarotene liposomes using high speed homogeniser method and to conduct an accelerated stability test on betacarotene liposomes produced using high speed homogeniser method. Methods: Nanoliposomes were prepared by high speed homogeniser method with time variation of 3, 6, 9 and 12 minutes and characterised for particle size, zeta potential value, polydispersity index, encapsulation efficiency, and stability. Results: The preparation of nanoliposomes produced a yellow, odourless suspension with antioxidant activity, with particle size characteristics measuring 200-300 nm, with a polydispersity index of 0.2-0.5 and potential zeta values ranging from -25 mV to -30 mV, encapsulation efficiency value of 97% and morphology of liposome particles in the form of spherical globules with 500x magnification. Conclusion: Formulation with a time variation of 12 minutes has recommended characteristics with spherical shape having good particle size, polydispersity index value, zeta potential value and encapsulation efficiency value.