Development of nano titania/polyvinylidene fluoride composite from new titanium(IV) derivative and its investigation on antibacterial, BSA interaction and cytotoxicity

Abstract

A new heteroleptic complex of titanium(IV) has been synthesised and characterized by physico-chemical techniques. Complex [(C36H30N4O4)Ti] 2a, was obtained by treating Ti(OPri)4 with a new modifier bidentate ligand 1a (C24H20N2O2) as well as with 8-hydroxy quinoline in 1:1:2 stoichiometry in anhydrous benzene. The 2a was employed as a precursor for the synthesis of active rutile phase nano titania by sol-gel method. Nano TiO2 was embedded with PVDF to obtain a set of three TiO2/PVDF samples of composite materials in diverse compositions (30 wt%, 40 wt% and 50 wt%). Ligand 1a, titanium(IV) derivative 2a, nano titania and trio samples of TiO2/PVDF composites were subjected for antibacterial studies against four infective microbial strains such as Bacillus cereus, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa by well diffusion technique. The 50 wt%-TiO2/PVDF composite material exhibited efficient antibacterial activity against all four pathogens. Protein binding interaction of Bovine Serum Albumin (BSA) with the third sample of composite material was performed and the cytotoxicity of the same composite was also tested against HeLa and HT- 29 tumor cell lines and was evaluated by MTT assay. The IC50 values of 50 wt%-TiO2/PVDF were observed as 35.72 µg/ml and 39.42 µg/ml on HeLa and HT-29 cell lines, respectively.