Development of nano-silver alkaline protease bio-conjugate depilating eco-benign formulation by utilizing potato peel based medium

Abstract

A new bio-conjugate nano-silver enzyme conjugate complex (BC-nAg-Akp) was formulated containing alkaline protease (Akp). The present research involved synthesis of nAg particles in acetone concentrated enzyme sol using 0.005 M AgNO3 solution formed within interaction time of 24 h through photo catalysis. The BC-nAG-Akp composite exhibited 1.9-fold increase in enzyme activity. The formulation was characterized using techniques viz., SEM, SEM-EDS, TEM, and DLS spectroscopy. The TEM analysis revealed synthesis of silver nano rods with size dimensions ranging from 40 to 80 nm. Likewise, the mean hydrodynamic diameter was 114 nm with polydispersity index of 0.260 and had the largest diffusion constant of 4.28 × 108 amongst the three forms of the formulation (crude, acetone concentrated and partially purified) on DLS characterization. The SEM-EDS analysis showed occurrence of 18.32 and 3.79%weight and %atom of Ag element respectively. The prepared formulation was investigated for its dehairing performance. The ideal dehairing was achieved at 37 °C after 12 h of treatment. The histopathological studies revealed that complete dehairing with minimal rarefication was achieved and was found perform better compared to the commercial Akp and control (crude enzyme) formulations.