Abstract
In wheat kernel, multiple gene loci, Psy-A1, Psy-B1, TaZds-A1, TaZds-D1, TaLox-B1, Ppo-A1 and Ppo-D1 are implicated in the expression of flour colour traits. In this study, eight functional markers, covering seven gene loci, were used to develop three multiplexed polymerase chain reaction (PCR) systems for marker-assisted selection (MAS) of desirable flour colour, i.e. PCR-I (YP7B-1/YP7B-2/YP2A-1), PCR-II (YP2D-1/PPO18) and PCR-III (YP7A/LOX18/STS01). Genotyping of 20 control wheat cultivars by these multiplex-PCR systems completely matched the results from the eight single PCR amplifications. Multiplex-PCR typing was successfully established for a total of 155 Chinese autumn-sown wheat cultivars. In multiplexed PCR-I, the occurring frequencies for three alleles (Psy-B1a, Psy-B1b and Psy-B1c) at the Psy-B1 locus were 33.5, 58.7, and 7.7%; while two alleles (TaZds-A1a and TaZds-A1b) at TaZds-A1 were 40.0 and 60.0%, respectively. In multiplexed PCR-II, the occurring frequencies, TaZds-D1a and TaZds-D1b (98....
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