Abstract
Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.
Highlights
Infection with enterotoxigenic Escherichia coli (ETEC) is one of the main causes of childhood diarrhea in developing countries and in travelers to these areas [7]
The labile toxin (LT) enterotoxin and the colonization factors (CFs) antigens are immunogenic, and one or both of these components are included in most vaccines that are being developed against ETEC diarrhea [14, 15]
To evaluate the putative impact of different vaccine compositions on ETEC diarrhea, it is important to characterize clinical ETEC isolates from different parts of the world with regard to toxin and CF profiles, since they have been observed to vary from one geographic region to another [4, 7, 8, 10, 12]
Summary
Infection with enterotoxigenic Escherichia coli (ETEC) is one of the main causes of childhood diarrhea in developing countries and in travelers to these areas [7]. Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections.
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