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Abstract
Objective:
 To determine the overexpress HGF gene in non HGF producing humane myeloma cell lines (INA-6).
 Materials and Methods:
 In this study, retroviral- expression constructs were designed and constructed. The sequence of the cloned fragments in the vector constructs was determined and the effect of construct was examined using qRT-PCR and Western blot analysis. At department of cancer research & molecular medicine, norwegien university of science & technology, Trondheim Norway, in June 2016 to April 2018.
 Results:
 We established expression constructs based on HGF mRNA sequence. The HGF non producing human myeloma cell line INA-6 transduced with lentiviral HGF-overexpression transduction particles, were analyzed for their effect on the HGF gene expression. HGF overexpression was analyzed both at mRNA level and protein levels. Two myeloma cell line INA-6 HGF-1 and INA-6HGF-2 showed significant over expression of HGF mRNA and protein.
 Conclusion:
 In conclusion, the HGF overexpression cell lines can be used as tissue culture models to investigate the role of HGF protein in pathology of multiple myeloma.
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