Development of multifunctional zein-based films engineered with gallic acid and Ag NPs loaded γ-CD-MOFs for pork preservation

Abstract

The increasing emphasis on food quality and consumer safety has prompted researchers to explore green manufacturing techniques for packaging materials. In this study, γ-cyclodextrin metal-organic frameworks (γ-CD-MOFs) were prepared as promising nanocarriers for gallic acid (GA) and silver nanoparticles (Ag NPs). These nanocarriers (GA-Ag@CD-MOFs) were further incorporated into a zein matrix to fabricate multifunctional composite films, referred to as MOFs-Zein. Scanning electron microscopy (SEM) and atomic force microscope (AFM) analysis indicated that the fabricated composite film exhibited excellent biocompatibility, with GA-Ag@CD-MOFs uniformly dispersed within the film matrix. Fourier transform infrared spectroscopy (FT-IR) result revealed that the incorporation of GA-Ag@CD-MOFs facilitated the formation of intermolecular hydrogen bonds with the matrix polymer. The addition of GA-Ag@CD-MOFs enhanced the tensile strength of the composite film by 19.70 %, improved the water barrier performance by 57.76 %, and provided almost 100 % UV protection. The exceptional controlled release capability of γ-CD-MOFs for GA empowered the composite films to maintain their robust initial antioxidant capacity even after 100 days of storage. Moreover, the composite films performed high-efficiency antibacterial activity against Escherichia coli O157:H7 and Salmonella enterica. Acute toxicological studies showed no histopathology related toxicity was found in GA-Ag@CD-MOFs treated mice. When applied to fresh pork, the composite films significantly reduced the key quality indicators of raw meat, compared to the control film. Overall, this work presents a promising approach to fabricate the composite films as a sustainable and functional solution for green functional food packaging.