Development of Multifunctional Cosmetic Cream Using Bioactive Materials from Streptomyces sp. T65 with Synthesized Mesoporous Silica Particles SBA-15.

Ram Hari Dahal,Jangyul Lee,Tuan Manh Nguyen,Jaisoo Kim,Dong Seop Shim,Joon Young Kim

doi:10.3390/antiox9040278

Abstract

Various cosmetics having a single function are increasingly being used, but cosmetics having multifunctional activities remain limited. We aimed to develop a multifunctional cosmetic cream having antioxidant, anti-tyrosinase, anti-aging and antimicrobial activities. Antimicrobial activities were performed by disc-diffusion method. Cell toxicity and cell proliferations were evaluated in a 96-well plate with different cell lines such as HaCaT, RAW264.7, CCD-986Sk, B16F1, and B16F10. Mushroom tyrosinase inhibition, elastase inhibition, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were evaluated and IC50 was calculated. Mesoporous silica particle was synthesized using Pluronic P123 and tetraethyl ortho-silicate (TEOS). Facial pictures were captured by VISIA-CR (Facial Imaging System for Clinical Research). Roughness of image was analysed by PRIMOS software and brightness of image was analyzed by Chromameter CR-400. The crude product of strain T65 inhibited the different human pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Propionibacterium acnes, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The IC50 of T65 crude product for mushroom tyrosinase, elastase, and DPPH radical scavenging activities were 58.73, 14.68, and 6.31 µg/mL, respectively. T65 crude product proliferated collagen type I in CCD-986Sk cell up to 145.91% ± 9.11% (mean ± SD; mean of 24, 48, and 72 h) at 250 pg/mL. Synthesized mesoporous particles (SBA-15) confirmed the sustainable performance by control-release for three days. Formulated functional cosmetic cream containing T65 embedded SBA-15, significantly decreased the skin roughness by 4.670% and increased the skin brightness by 0.472% after application of 4 weeks. T65 crude product inhibited both Gram-positive and Gram-negative pathogens. Synthesized mesoporous particle, SBA-15, confirmed the physiologically active substance was released in sustainable release condition. T65 crude product showed impeccable antimicrobial, antioxidant, anti-aging, and whitening activities with non-cytotoxic effects to different cell lines related to the human skin.

Highlights

The skin is the largest organ and outer covering of the human body
Elastin helps skin to return to its original position after movement, whereas elastase produced in acinar cells degrades the elastin and leads to skin aging [9]
Secondary bioactive materials extracted from the soil microorganisms are of interest for cosmetic

Summary

Introduction

The visual appearance of the skin allows for estimation of age, gender, health, attractiveness, and beauty [1,2]. Cosmetic products are extensively used for enhancing the appearance of the skin and to reduce skin aging. Skin aging is a complex biological process caused by various intrinsic and extrinsic factors that lead to physiological dysfunction and loss of the structural integrity of the skin. Generally known as natural aging (chronologic aging) of the skin is related to hormonal changes based on age, whereas extrinsic aging is associated with movement of the muscle, pollution, nicotine, exposure to solar radiation, caffeine, temperature, lifestyle such as diet (nutrition), lack of sleep, stress, and other health conditions [7,8,9]. Cosmetic or skin care products with anti-aging properties positively affect skin aging [10,11,12,13]

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