Development of Moxifloxacin Hydrochloride loaded in-situ gel for the treatment of periodontitis: In-vitro drug release study and antibacterial activity

Abstract

Periodontitis is one of the most widespread oral diseases. Medicated in-situ gels of Moxifloxacin Hydrochloride for extended period of retention in infected cavity were prepared for improved local action for the treatment of periodontitis. Medicated formulations were prepared using temperature sensitive (poloxamer 407), ion sensitive (gellan gum) and pH sensitive (carbopol 934P) polymers. 32 Full Factorial Design has been applied and prepared batches were characterized by FTIR, pH, syringeability, drug content, clarity, gelation temperature, gelling time, in-vitro gelling capacity, in-vitro diffusion study. Gelation temperature, (in-vitro) gelling time and the nature of gel formed in simulated saliva showed polymeric concentration dependency. Diffusion study of in-situ gel had been performed which showed augmented arrival of medication from 7-12 hours and the discharge was dependent on polymer utilized. The best fitted model was zero order kinetics which indicated that the formulation gave controlled delivery. All preparations were non-Newtonian and display pseudoplastic conduct. Invitro Antimicrobial study was carried out by utilizing E. coli and S. aureus. Optimized formulation containing 19.072 %w/v poloxamer 407 and 0.245 %w/v gellan gum exhibited desired characteristics for developing periodontal drug delivery systems.