Abstract
Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate synergism, ethanol extract and hexane fraction were also added to formulations containing 0.05% sodium fluoride and 0.12% chlorhexidine digluconate. These formulations were assessed for inhibitory effect against the specific microorganisms involved in the process of bacterial plaque formation, S. mutans(ATCC25175) and C. albicans(ATCC 10231), frequently found in cases of oral infections. The agar diffusion method was used to evaluate the inhibitory activity of extracts and formulations. All mouthwash solutions displayed inhibitory activity having higher sensitivity to S. mutansfor the 5% ethanol extract+0.05% sodium fluoride, and greater sensitivity to C. albicansfor the 10% hexane fraction. Results were characterized by the appearance of a growth inhibition halo, justifying the utilization and association of extracts of R. officinalis.
Highlights
Rosmarinus officinalis belonging to the family Lamiaceae and popularly known as rosemary, has been widely used in folk medicine (Silva et al, 2008; Tressino, Gabriel, 2009)
The aim of this study was to evaluate the antimicrobial activity of the extract and fractions of Rosmarinus officinalis, developing a mouthwash formulation with its extract, and to evaluate its synergism with substances currently used against dental biofilm
The study of antimicrobial activity by the diffusion method showed that the ethanol extract and its fractions were effective in inhibiting the growth of S. mutans and C. albicans (Table III)
Summary
Rosmarinus officinalis belonging to the family Lamiaceae and popularly known as rosemary, has been widely used in folk medicine (Silva et al, 2008; Tressino, Gabriel, 2009). Studies suggest potential of the antimicrobial effect of R. officinalis as a treatment for oral diseases (Bugno et al, 2006; Alvarenga et al, 2007; Bernardes et al, 2010; Maekawa et al, 2010). The aim of this study was to evaluate the antimicrobial activity of the extract and fractions of Rosmarinus officinalis, developing a mouthwash formulation with its extract, and to evaluate its synergism with substances currently used against dental biofilm
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