Development of Moringa oleifera Standardized Leaf Extract Nanofibers Impregnated onto Hydrocolloid Film as A Potential Chronic Wound Dressing

Abstract

This research focuses on the development of a hybrid dressing, i.e. Moringa oleifera (MOL) leave extract nanofibers impregnated onto an alginate-pectin hydrocolloid film for chronic wound healing. The MOL nanofibers impregnated hydrocolloid films were characterised and optimised by physicomechanical properties, moisture transmission, swelling ratio, microscopy analysis, Franz diffusion drug release and cell viability assays. From the preformulation studies, PEO 6 % solution with viscosity above 1.3 Pa·s was found suitable to be electrospun into uniform nanofibers (diameter 322.7±78.02 nm). The moisture transmission rate and swelling ratio studies revealed that the MOL nanofiber-film was useful for the application of light to medium suppurating wounds. The Franz diffusion cell study showed that the electrospun duration could influence the amount of MOL released from the nanofibers-film. Finally, cell viability assays revealed that the MOL nanofibers-films hybrid dressing did not cause cytotoxicity to human dermal fibroblast and human epithelial keratinocytes cell lines. In conclusion, a hybrid MOL nanofibers impregnated hydrocolloid films dressing was successfully formulated and this study has implied that MOL nanofibers-film could be an alternative treatment option for treating chronic wounds.