Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis.

Bok Kyung Ku,Haryoung Poo,Hyun Mi Pyo,Jae-Won Byun,Jinju Nah,Kang-Seuk Choi,Quyen Thi Nguyen,Mi-Young Park,Jihyun Yang,Soyoon Ryoo,Sung-Hwan Wee

doi:10.3390/pathogens8040301

Abstract

Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O1 Manisa). Importantly, the solid-phase competitive ELISA (SPCE) using horseradish peroxidase (HRP)-conjugated #106 mAb detected FMDV type A-specific Abs in sera from FMD type A-vaccinated cattle more effectively than a commercial SPCE. These results suggest that the newly developed FMDV type A-specific mAb might be useful for diagnostic approaches for detecting Abs against FMDV type A.

Highlights

Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals and is caused by infection with an FMD virus (FMDV), a member of the genus Aphthovirus in the familyPicornaviridae [1]
The solid-phase competitive ELISA (SPCE) using a horseradish peroxidase (HRP)-conjugated #106 monoclonal antibodies (mAbs) more effectively detected FMDV type A-specific Abs in the sera from FMDV type A-vaccinated cattle compared to a commercial SPCE
To generate anti-FMDV type A mAbs, we immunized the footpads of BALB/c mice with inactivated

Summary

Introduction

Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals and is caused by infection with an FMD virus (FMDV), a member of the genus Aphthovirus in the familyPicornaviridae [1]. Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals and is caused by infection with an FMD virus (FMDV), a member of the genus Aphthovirus in the family. FMD outbreaks result in a devastating impact on economies due to constraints on the international trade of livestock and animal products [2,3,4]. FMDV exists in seven distinct serotypes comprising O, Asia 1, A, C, and South African territory (SAT) 1, 2, and 3 [5,6]. FMDV serotypes worldwide, and FMD type A outbreaks occur in many countries, including South. An inactivated FMD vaccine using a predominant FMDV type A strain, A22/Iraq/1964, has been widely used for preventing FMDV type A infections [8,9,10]

Methods

Results

Discussion

Conclusion