Abstract
A new, simple, rapid, and sensitive solid-phase extraction with molecularly imprinted polymer (SPE-MIP) for determination of Carbamazepine in biological samples was developed. These nano particle polymers were synthesized via a non-covalent molecular imprinting approach through precipitation polymerization method using methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker agent, carbamazepine as a target template molecule and azobisisobutyronitrile (AIBN) as an initiator. The optimal conditions for SPE consisted of conditioning the cartridge using a pH=3.0 water, loading 5.0 ml of the sample under basic aqueous conditions, clean-up using 2×2ml acetonitrile and elution with 3.0 ml methanol. After optimization of SPE procedure, an aliquot of extracted template was injected to the ACE, 5μm 250×4.6 mm analytical column with the mobile phase as the same as elution solvent. This method was used for extraction of carbamazepine from an anticonvulsant aqueous solution and the results revealed an extraction recovery of more than 90%. HPLC chromatograms show an efficient clean up, which supports the potential of MISPE for clean-up of trace amounts of carbamazepine from the drug formulation.
Highlights
Introduction the popularity ofSolid-phase extraction (SPE), new solid sorbents have appearedCarbamazepine (CBZ) is widely used as the first line as alternative to conventional sorbents with the aim of treatment for partial and generalized Tonic–clonic seizures achieving more and more selective preconcentration of (Beghi, 2002)
The materials routinely used in SPE are concentrations in epileptic patients
Imprinted polymers (MIPS) offer the concentration depend to different factors, which affect possibility of achieving selective extraction, analogous to pharmacokinetics of the drug (Bertilsson & Tomson, 1986). those achieved by immuno-based extraction systems, Because of abnormalities in neurotransmitters, and may represent an advance on conventional SPE
Summary
The molecular imprinting technique has emerged as Methacrylic acid (MAA) from Merck (Darmstadt, a powerful approach for the creation of recognition site in Germany) was distilled in vacuum prior to use in order to highly cross-linked polymeric matrices. Carbamazepine reference to the print molecule structure These imprints exhibit standard was used for preparing stock and standard selectivity for rebinding the template with which it was prepared. These MIPS nano particles have been employed in fields where a certain degree of selectivity is solutions. For the preparation of the carbamazepine imprinted polymer nano particles, the template (94 mg, 0.25 mmol) was dissolved in asetonitril in a 25 mL thick walled glass tube. After the polymerization procedure and drying, the polymer nano particles were washed with methanol and acetic acid (10:1, v/v, of 98% methanol and pure acetic acid) for three times and with distillated water for two times. All of the analyses were carried out at an operation wavelength of 225 nm
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