Development of molecular markers for genetic male sterility in Gossypium hirsutum

Xuehui Feng,Scott Huesgen,Don Keim,Humphrey Wanjugi,Seungho Cho,Issa Coulibaly,Yan Fu,John Schwarz

doi:10.1007/s11032-015-0336-z

Xuehui Feng, Scott Huesgen + Show 6 more

Open Access

https://doi.org/10.1007/s11032-015-0336-z

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Abstract

Genetic male sterility (GMS) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. Sequence analysis on ms5 and ms6 loci in Gossypium hirsutum was conducted to reveal genomic variation at these two loci between GMS and wild-type G. hirsutum inbred lines, and sequence polymorphism linked to ms5 on A12 and ms6 on D12 was revealed. A haplotype marker set that consisted of four SNPs targeting both ms5 and ms6 gene regions was developed and validated for association with GMS in cotton. Predictability of GMS phenotype by this haplotype SNP set was over 99 %. GMS haplotype marker set can serve as a high-throughput molecular breeding tool to select GMS individuals and improve hybrid production efficiency.

Highlights

Genetic male sterility (GMS) in cotton occurs in a form of non-dehiscent anthers and unviable pollens when reproductive development process fails due to possible loss of nuclear gene functions required for pollen development
Genes related to GMS phenotypes can be transferred to various genetic backgrounds by routine crossing practice and allow the trait to be inherited at full penetrance in successive generations
Because GMS maintainers need to be fertile, recessive genetic mechanisms with complete male sterility especially mediated by ms5 and ms6 have been widely used in hybrid cotton production (Basu 1996)

Summary

Introduction

Genetic male sterility (GMS) in cotton occurs in a form of non-dehiscent anthers and unviable pollens when reproductive development process fails due to possible loss of nuclear gene functions required for pollen development. Single recessive genes ms and ms reportedly conferred partial genetic male sterility (Justus and Leinweber 1960; Justus et al 1963). GMS conferred by a single recessive gene, ms, and paired recessive genes, ms and ms, conferred stable male sterility (Richmond and Kohel 1961). Because GMS maintainers need to be fertile, recessive genetic mechanisms with complete male sterility especially mediated by ms and ms have been widely used in hybrid cotton production (Basu 1996)

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