Abstract
A mobile laboratory transportable on commercial flights was developed to enable local response to viral hemorrhagic fever outbreaks. The development progressed from use of mobile real-time reverse-transcription polymerase chain reaction to mobile real-time recombinase polymerase amplification. In this study, we describe various stages of the mobile laboratory development. A brief overview of mobile laboratory deployments, which culminated in the first on-site detection of Ebola virus disease (EVD) in March 2014, and their successful use in a campaign to roll back EVD cases in Conakry in the West Africa Ebola virus outbreak are described. The developed mobile laboratory successfully enabled local teams to perform rapid disgnostic testing for viral hemorrhagic fever.
Highlights
MethodsThe development progressed from use of mobile real-time reverse-transcription polymerase chain reaction to mobile real-time recombinase polymerase amplification
A mobile laboratory transportable on commercial flights was developed to enable local response to viral hemorrhagic fever outbreaks
A brief overview of mobile laboratory deployments, which culminated in the first on-site detection of Ebola virus disease (EVD) in March 2014, and their successful use in a campaign to roll back EVD cases in Conakry in the West Africa Ebola virus outbreak are described
Summary
The development progressed from use of mobile real-time reverse-transcription polymerase chain reaction to mobile real-time recombinase polymerase amplification. We describe various stages of the mobile laboratory development. In ML1–2 stages, for qRT-PCR, we chose the SmartCycler (Cepheid, Sunnyvale, CA) [16, 17], which was used by other teams [18,19,20,21]. In ML3–4a/b stages, for RT-RPA, we used the Twista (TwistDx, Cambridge, UK) and the TS2.2 device (QIAGEN Lake Constance, Stockach, Germany). In ML1–3, electricity was tapped from a motor vehicle battery via inverter (HPL 1200-D-12 inverter, 12V, 1200W). In ML3, electricity was tapped via solar panel and power pack Yeti 1250. In ML4, electricity was tapped via solar panel and power pack Yeti 400 (GOALZERO, Bluffdale, UT) (Table 1)
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