Abstract
The early development of eigth lymphocyte subsets was determined for pigtailed macaque infants from 0 to 800 days of age using two-color flow cytometry and fluorescein- and R-phycoerythrin-conjugated monoclonal antibodies specific for human leukocyte antigens. Four major lymphocyte subsets in monkeys (B, CD4 + T, and NK cells) could be further divided using two-color analysis. In neonates, the frequency of lymphocyte subpopulations having surface phenotypes found principally on dense, resting cells (IgD + B cells, Lp220 + CD4 + T cells, and CD18 dull CD8 + cells) was much higher than subpopulations having phenotypes present principally on buoyant, activated cells (IgD − B cells, Lp220 − CD4 + T cells, CD18 bri CD8 + T cells). There was a complete absence of two CD18 bri CD8 + subsets (CD8 dull and CD8 bri) during first 300 days of life. The relative proportion of lymphocyte subsets with resting phenotype decreased with increasing age, while the subpopulations associated with activation gradually increased with age. These findings suggest that during early development immunocompetent cells gradually differentiate into activated lymphocytes.
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