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Abstract
In this study, a high sensitive and reproducible method based on immunoaffinity cleanup followed by ultra-performance liquid chromatography-tandem mass spectrometry has been developed for the simultaneous determination bisphenol A (BPA) and bisphenol S (BPS) in aquatic products. The immunoaffinity column was prepared by coupling N-Hydroxysuccinimide-activated Sepharose 4B gel with monoclonal antibodies of BPA and BPS, which can selectively recognize the target analytes to achieve a low matrix interference. The matrix effects of different aquatic products were all less than ±10%. The limits of detection in four kinds of aquatic products was 0.060 μg/kg for BPA and 0.012 μg/kg for BPS. The recoveries of BPA and BPS in matrices varied from 74% to 106% with relative standard deviations <12%. The proposed method was successfully applied to real aquatic products.
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