Abstract
Development of spontaneous synchronized bursting in the early stages of rat neocortical neuronal cultures was studied by whole-cell and extracellular recordings. Neocortical neurons from rat embryos were cultured on planar electrode arrays, and low Mg2+-induced spontaneous activity was recorded from 5 to 16 days in vitro (DIV). At 5–6 DIV the current synchronized to the bursting had only a slow component lasting 3–5 s, whereas in older cultures a fast transient component was dominant. A γ-aminobutyric acid (GABA)A receptor antagonist, bicuculline methiodide, had little effect on the spontaneous activity at 5–6 DIV, whereas in older cultures it had a marked effect on the slow current component. These results suggest a role of GABAergic transmission in the development of synchronized activities.
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