Abstract
Summary Responsiveness of ob17 cells to lipolytic agents was examined by cAMP production and by fatty acid release in the presence of isoproterenol. EC50 values for cAMP production increased from 70 nM (growth phase) to 120 nM (resting phase), with a maximal response at 2.5 μM. Maximal responsiveness was already present in subconfluent cells, was magnified 3 fold when the cells attained the confluent state and subsequently decreased as a function of time in culture. In contrast early confluent ob17 cells, containing triglycerides prelabeled in their fatty acid moiety, did not show any significant lipolytic response at 2.5 μM isoproterenol. A full lipolytic response was observed in differentiated ob17 cells, indicating that the most critical event during adipose conversion for lipolysis occurs at a step beyond the β-adrenergic-adenyl cyclase coupling. Chronic exposure of post-confluent ob17 cells to insulin led to a diminished maximal responsiveness to isoproterenol, suggesting the establishment of an alteration on a long term basis to the action of β-agonists.
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