Abstract
This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 μg L−1 and 60 μg kg−1, and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 μg L−1 and 40 μg kg−1, respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B1, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B1. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples.
Highlights
Zearalenone (ZEN), known as F-2 toxin, is a secondary metabolite produced by Fusarium, which mainly contaminates corn, wheat, and other cereals [1,2]
The UV-visible spectra of Au nanorods (AuNRs) indicated that the centers of the transverse and longitudinal SPR peaks are located at 541 nm and 850 nm, respectively
This study has developed two kinds of visual immunoassay test strips based on the colloidal
Summary
Zearalenone (ZEN), known as F-2 toxin, is a secondary metabolite produced by Fusarium, which mainly contaminates corn, wheat, and other cereals [1,2]. ZEN has a similar structure with the endogenous estrogen and can competitively bind to the estrogen receptor, which makes the hormone of animal body maladjusted and destroys the reproductive system [3,4]. The contamination range of ZEN in grain is very wide. It cannot only enter the human body through direct or indirect intake, but can be transmitted to the fetus through placenta. Modern research has confirmed that ZEN has typical immunotoxicity, genotoxicity, and reproductive toxicity, which seriously threatens people’s health [5,6,7]. The development of effective methods for ZEN detection in various foods is of great significance for protecting the health of people
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