Development of inter-varietal chromosome substitution lines of wheat (Triticum aestivumL.) bearing a rye genetic marker and their verification with microsatellite markers

Abstract

Two monosomic alien substitution lines (MAS lines, 2n=41=40+5R) of wheat Triticum aestivum L. cv. ‘Saratovskaya 29’ were used as recipients in the development of inter-varietal substitution lines for chromosomes 5A and 5D. In the MAS lines, chromosomes 5A or 5D of ‘Saratovskaya 29’ were replaced by homoeologous univalent chromosome 5R of rye Secale cereale L. cv. ‘Onokhoiskaya’, which bears the Hp marker gene coding for hairy peduncle. The donors included 18 spring and winter wheat varieties. The MAS lines were developed by crossing monosomic lines of ‘Saratovskaya 29’ for chromosomes 5A and 5D to a wheat-rye substitution line of ‘Saratovskaya 29’ 5R(5D) followed by cytological and morphological selection of plants with chromosome configuration 20II +5RI in metaphase I of pollen mother cells from F 1 and F 2 plants with slightly hairy peduncles. It was shown that MAS lines could be maintained during long-term propagation (18 generations). Use of MAS lines with the Hp marker gene allows acceleration and abbreviation of cytological analysis and elimination of the probability of ‘univalent switch’ in the course of the development of substitution lines. The method was applied to the development of 22 ‘Saratovskaya 29’ lines with inter-varietal substitution for chromosomes 5A and 5D. Fourteen and thirteen microsatellite markers located in chromosomes 5A and 5D, respectively, were used to prove the authenticity of the inter-varietal substitution lines. According to these markers, 21 substitution lines from 22 studied were correct.