Abstract
A new method developed for in-vitro susceptibility test in medical laboratories consist of micro tubes or gloves containing dehydrated tryptic soya broth, 5% glucose, 0.1% bromothymol blue and one type of antibiotics (ampicillin, tetracycline and chloramphenicol) with critical concentration MIC (minimum inhibitory concentration) for susceptibility. Standard quality control strains of bacterial (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) suspension were adjusted to 0.5 McFarland turbidity standard (1 × 106 cell/mL) were used in inoculation the media and incubated two hours at 37 °C. The MIC of ampicillin against E. coli, S. aureus, and P. aeruginosa were 4, 32, and 256 µg/mL of the media for the bacteria respectively, while the MIC of tetracycline against bacteria were 512, 512 and 32 µg/mL respectively, the MIC of chloramphenicol were 512, 32 and 512 µg/mL, respectively. Where, the resistant bacteria to the antibiotics could grow and ferment glucose sugar producing a color change of the media from blue to yellow, while the sensitive bacteria do not grow or show no change in color. Our study result compared with common used antibiotic disk method obtaining similar results. This developed method characterized by fast (only two hours) and less cost in comparison to conventional technique. The new micro tube strip is highly stable (more than one year) with more sensitive in detection of variable pathogenic bacteria including standard bacteria strains compared with conventional technique..
Highlights
The world we live in is full of synthetic chemicals, most of which are toxic
While two pathogenic fungal genera, Microsporum gypseum isolated from patient with dermatophyte infection, and Aspergillus flavus isolated from the soil are identified depending on macroscopic and microscopic examination of the culture isolates [6, 7]
From the same table different inhibitory effects can be observed, Philodendron bipinnatifidum and Aglonema commutatum exhibit more inhibitory effect than Ficus elastica which may be due to the difference in the amount of active inhibitory compounds excreted by each plant
Summary
The world we live in is full of synthetic chemicals, most of which are toxic. They are encountered from our food to all the objects we touch. The plates are inoculated with test bacteria by a sterile cotton swabs which dipped into diluted bacterial suspension (10-4), using a micropipette 200 μL of the solution is added to these pores and the plates are incubated at 37 °C for 18-24 h the diameter of inhibition zones was measured in millimeter using a ruler [8].
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