Development of in vitro protocol for mass multiplication of open pollinated seedling of rose (Rosa × hybrida L.) cv. Rose Sherbet

Abstract

An in-vitro protocol for promising open pollinated seedling of rose (Rosa × hybrida L.) cv. Rose Sherbet was developed for mass multiplication. Murashige and Skoog (MS) medium supplemented with 5.0 mg/l BAP + 0.1 mg/l NAA+ 0.5 mg/l GA3 was found most effective for culture establishment. The maximum number of micro shoots (36.67 shoots/explant) were induced on MS medium comprising 5.0 mg/l BAP + 0.1 mg/l NAA+ 0.5 mg/l GA3 along with 40 mg/l adenine sulphate and was found to be better for shoot proliferation. The highest shoot length after 15 days (1.90 cm), 30 days (3.25 cm) and 45 days (4.65 cm) was observed on MS medium supplemented with 1.0 mg/l GA3. Rooting of micro shoots was successfully induced on half strength MS basal medium supplemented with NAA (0.5 mg/l) rooting growth regulators. The regenerated plantlets were efficiently hardened in glass jars filled with coco peat + vermiculite + perlite (1: 1: 1) moistened with half strength MS medium salts and covered with polypropylene lids, thereafter plants were successfully transferred to the field with good survival.