Abstract
Over the past few years, image analysis has emerged as a powerful tool for analyzing various cell biology parameters in an unprecedented and highly specific manner. The amount of data that is generated requires automated methods for the processing and analysis of all the resulting information. The software available so far are suitable for the processing of fluorescence and phase contrast images, but often do not provide good results from transmission light microscopy images, due to the intrinsic variation of the acquisition of images technique itself (adjustment of brightness / contrast, for instance) and the variability between image acquisition introduced by operators / equipment. In this contribution, it has been presented an image processing software, Python based image analysis for cell growth (PIACG), that is able to calculate the total area of the well occupied by cells with fusiform and rounded morphology in response to different concentrations of fetal bovine serum in microfluidic chips, from microscopy images in transmission light, in a highly efficient way.
Highlights
Since the beginning of cell biology, scientists have sought methods to isolate and cultivate different cell lines for the investigation of cell and dynamics biology and their subsequent clinical application [1]
fetal bovine serum (FBS) was constituted as a standard supplement of the cell culture medium, which is obtained and contains a high concentration of growth factors and a low concentration of gammaglobulins, compared to other sera originated from animals [4]
Python based image analysis for cell growth (PIACG) has been used to analyze the effects of increasing concentrations of FBS on HEK-293T cell line during 10 days of cell culture
Summary
Since the beginning of cell biology, scientists have sought methods to isolate and cultivate different cell lines for the investigation of cell and dynamics biology and their subsequent clinical application [1]. Special combinations of nutrients are required in the culture media to provide optimum conditions for the survival and in vitro growth of the different cell lines under study [2]. To maintain cell function and allow cell division and proliferation, the culture medium is universally complemented with fetal bovine serum (FBS), a mixture containing growth factors among its components [3]. FBS was constituted as a standard supplement of the cell culture medium, which is obtained and contains a high concentration of growth factors and a low concentration of gammaglobulins, compared to other sera originated from animals [4].
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