Development of HPTLC method for estimation of piperine, guggulsterone E and Z in polyherbal formulation

Abstract

AimTriphala guggul a polyherbal tablet formulation is used for sinusitis, allergies, boils, constipation, piles, high cholesterol, rheumatism, mal-absorption, purgative and as blood purifier. In the present study an attempt has been made to develop a simple, precise, rapid and cost-effective high performance thin-layer chromatographic (HPTLC) method for quantitative estimation of piperine, guggulsterone E and Z in Triphala guggul formulation. MethodThe different batches of formulation were prepared in laboratory by using authenticated raw material and were subjected to various physical and chemical evaluations. Then the prepared formulation and three commercial formulations were investigated for the qualitative and quantitative estimation of mentioned constituents. The methanolic extract of all formulations were quantified by using HPTLC studies. Linear regression data for the calibration curves of standards viz. piperine, guggulsterone E and Z showed a good linear relationship over a concentration range of 0.06–0.14 μg/spot, 2.5–17.5 μg/spot, 5–30 μg/spot respectively with the correlation coefficient of 0.99085, 0.99847, 0.9990 respectively and thus exhibits good linearity between concentration and area. The content of guggulsterone E (14.68 %w/w, 13.05 %w/w, 6.36 %w/w, 14.36 %w/w); guggulsterone Z (31.81 %w/w, 26.95 %w/w, 11.62 %w/w, 23.86 %w/w); and piperine (0.068 %w/w, 0.0150 %w/w, 0.321 %w/w, 0.0375 %w/w) were found in TF, TN, TM, TP prepared and three marketed formulation respectively. ConclusionThe proposed HPTLC method was found to be rapid, simple and linear for quantitative estimation of piperine, guggulsterone E & Z in different formulations and extracts.