Development of Highly Sensitive LC–MS and CE–MS Methods for In-Depth Proteomic and Glycomic Profiling of Limited Biological Samples

Abstract

nformative and deep proteomic and glycomic characterization of limited availability biological and medical samples has been a significant challenge. Here, we describe our current and recent efforts in advancing sample preparation as well as miniaturized electric field- and pressure-driven separation approaches interfaced with high-end mass spectrometry (MS) to enhance the sensitivity and depth of proteomic and glycomic profiling of several types of limited biological and clinically relevant samples.