Abstract
The rapid and sensitive detection of Staphylococcus aureus (S. aureus) is essential to ensure food safety and protect humans from foodborne diseases. In this study, a sensitive and facile electrochemical immunosensor using AuPdPt trimetallic nanoparticles functionalized multi-walled carbon nanotubes (MWCNTs-AuPdPt) as the signal amplification platform was designed for the label-free detection of S. aureus. The nanocomposite of MWCNTs-AuPdPt was prepared by an in situ growth method of loading AuPdPt trimetallic nanoparticles on the surface of MWCNTs. The synthesized MWCNTs-AuPdPt featured good conductivity and superior catalytic performance for hydrogen peroxide. The nanocomposite of MWCNTs-AuPdPt with good biocompatibility and high specific surface area was further functionalized by anti-S. aureus antibodies. The immobilized antibodies could efficiently capture S. aureus to the modified electrode by an immune reaction, which resulted in the change of catalytic current intensity to realize the sensitive detection of S. aureus. The designed immunosensor could detect S. aureus in a linear range from 1.1 × 102 to 1.1 × 107 CFU mL−1 with a low detection limit of 39 CFU mL−1. Additionally, the proposed immunosensor was successfully applied to determine S. aureus in actual samples with acceptable results. This strategy provided a promising platform for highly sensitive determination of S. aureus and other pathogens in food products.
Highlights
S. aureus is recognized for its serious pathogenicity and has become an important source of many diseases, ranging from superficial skin infections to life-threatening systemic diseases such as abscesses, endocarditis, meningitis, and bacteremia [2,3,4]
X-ray photoelectron spectroscopy (XPS) was further introduced to analyze the elemental composition of multi-walled carbon nanotubes (MWCNTs)-AuPdPt
The above results were in agreement with previous reports [24,25]. These results further indicated that AuPdPt trimetallic nanoparticles were successfully modified on the surface of MWCNTs
Summary
Food safety as a major public health issue has currently attracted widespread attention, which is related to ensuring healthier lives as well as protecting the national economy. Staphylococcus aureus (S. aureus), a typical and dangerous foodborne pathogen, can produce a number of toxins that cause damage to biological membranes by interacting with the host, leading to cell death [1]. S. aureus is recognized for its serious pathogenicity and has become an important source of many diseases, ranging from superficial skin infections to life-threatening systemic diseases such as abscesses, endocarditis, meningitis, and bacteremia [2,3,4]. It is urgent to establish a sensitive and specific method for the detection of S. aureus in many fields such as food safety, medical diagnosis, and public health management
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