Abstract
Given the low substitution rate in plastomes, the polymorphic and codominant nature of chloroplast SSRs (cpSSRs) makes them ideal markers, complementing their nuclear counterpart. In Cupressaceae, cpSSRs are mostly paternally inherited, thus, they are useful in mating systems and pollen flow studies. Using e‐PCR, 92 SSR loci were identified across six Cupressaceae plastomes, and primers were designed for 26 loci with potential interspecific transferability. The 26 developed cpSSRs were polymorphic in four genera, Platycladus, Sabina, Juniperus, and Cupressus and are suitable for Cupressaceae molecular genetic studies and utilization. We genotyped 192 Platycladus orientalis samples from a core breeding population using 10 of the developed cpSSRs and 10 nuclear SSRs, and these individuals were identified with high confidence. The developed cpSSRs can be used in (1) a marker‐assisted breeding scheme, specifically when paternity identification is required, (2) population genetics investigations, and (3) biogeography of Cupressaceae and unraveling the genetic relationships between related species.
Highlights
| MATERIALS AND METHODSTo evaluate the polymorphism and interspecific transferability of the chloroplast SSRs (cpSSRs) markers with the designed primers, we sampled 48 individual trees representing Platycladus orientalis (n = 24), Sabina chinensis (n = 8), Juniperus formosana (n = 8), and Cupressus torulosa (n = 8)
This study presents a set of polymorphic chloroplast SSRs (cpSSRs) markers which are transferable across diverse genera of Cupressaceae and demonstrates their value for genetic discrimination and diversity studies in this family
The cpSSRs found in this study are valuable genetic markers for its high degree of interspecific transferability and polymorphism and encourage the use of cpSSRs in population genetic research and coniferous tree breeding
Summary
To evaluate the polymorphism and interspecific transferability of the cpSSR markers with the designed primers, we sampled 48 individual trees representing Platycladus orientalis (n = 24), Sabina chinensis (n = 8), Juniperus formosana (n = 8), and Cupressus torulosa (n = 8). Initial screening via electrophoresis showed that amplicons were obtained for 24 of the 26 e-PCR-selected primer pairs, and amplification of two primer pairs (N14 and N23) was detected only in P. orientalis, S. chinensis, and J. formosana, and not in C. torulosa. In the second screening step, the amplicons of most of the primer pairs were monomorphic in the C. torulosa, while most of pairs showed polymorphism in J. formosana samples These results may be due to the different degree of genetic variation among the samples. TA B L E 2 Primer pairs of 26 cpSSRs designed by mining the plastomes of six species
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
