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Abstract
Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants. 
 Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline.
 Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a densitometric analysis of high-performance thin layer chromatography (HPTLC). Silica gel 60 F254 glass-backed plates (E-Merck, Germany, 0.2 mm layers) as stationary phase and mixture n-hexane: ethyl acetate 8 : 2 (%, v/v) as mobile phase were used to produce a sharp, symmetrical and well-resolved peak at an Rf value of 0.19 ± 0.02 and 0.52 ± 0.04 for α-pinene and eucalyptol, respectively. Linearly range for α-pinene was 100–700 ng/spot (r2 = 0.9988), whereas that for eucalyptol was 1000–7000 ng/spot (r2 = 0.9987).
 Conclusion: The developed method was found to be a simple, accurate, and precise, and it may be used to simultaneously analyses of many medicinal plants samples containing α-pinene and eucalyptol.
Highlights
The extracts and essential oils of M. longifolia, S. officinalis and R. officinalis are widely used as functional ingredients for therapeutics, flavoring, condiments, and food and nutraceutical industries [1,2]
Visible light spectra measured for the bands showed maximum absorbance at 500 nm for both α-pinene and eucalyptol respectively
The peak areas against the amount of α-pinene and eucalyptol were linear in the range of 100– 700 ng/spot and 1000-7000 ng/spot respectively
Summary
The extracts and essential oils of M. longifolia, S. officinalis and R. officinalis are widely used as functional ingredients for therapeutics, flavoring, condiments, and food and nutraceutical industries [1,2]. Gas chromatography and mass spectroscopy (GC-MS) analysis have been already evaluated for the presence of both αpinene and eucalyptol in the leaves of M. longifolia [3], S. officinalis [4] and R. officinalis [5]. Α-pinene has been evaluated for pharmacological activities such as bronchodilator, antioxidant, hypoglycemic, antimicrobials, anti-inflammatory, sedative, and gastro protective activities [11]. Αpinene has been approved as a safe food additive by the U.S FDA and it is widely used as a flavoring ingredient in several food products [12]
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