Abstract
Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly sensitive and specific for AIV detection, and much practical and economic for test-in-field or onsite. Many such assays have been developed and are still in developing since the H5N1 highly pathogenic AI (HPAI) outbreaks occurred in South East Asia in 2003. A MAb-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed in our lab during late 1990s and early 2000s. Meanwhile, AIV H7 and H5 subtype specific-MAbs have been successfully developed in our laboratory to enhance the Dot-ELISA and other MAb-based assays for AIV detection. Production and purification of the H7 and H5 MAbs were made to provide essential reagents for Dot-ELISA and other immunoassays, and the current development of a novel Biosensor technique for rapid detection of AIV from clinical and field specimens.
Highlights
The H5N1 highly pathogenic avian influenza (HPAI) outbreaks occurred initially in domestic poultry and wild birds species in Southeast Asian in 2003 and soon spread to Middle East, Europe and Africa, and currently are still ongoing in poultry in a number of countries
This paper briefly describes the development and production of AI viruses (AIV) H5 subtype-specific Monoclonal antibodies (MAb) to enhance MAb-based assays (e.g., Dot-enzyme-linked immunosorbent assay (ELISA) and Biosensor) for the rapid detection of AIV
Findings of MAb evaluation studies indicate that these MAb are highly sensitive and specific for the detection of their homologous subtypes of AIV by MAbs-based Dot-ELISA, indirect fluorescent antibody (IFA) test, immunohistochemistry (IHC) and Biosensor assay (Table 1)
Summary
The H5N1 highly pathogenic avian influenza (HPAI) outbreaks occurred initially in domestic poultry and wild birds species in Southeast Asian in 2003 and soon spread to Middle East, Europe and Africa, and currently are still ongoing in poultry in a number of countries. Because all subtypes of AI viruses (AIV) or influenza A viruses, H7 and H5 subtypes, have the ability to mutate from a low pathogenic strain to a high pathogenic strain. If they are allowed to circulate in poultry flocks, they have the ability to become highly lethal [2,3,4], and the HPAI H5N1 virus could be eventually mutated to a human pathogen being able to infect humans more and spread from one person to another. MAb-based diagnostic assays are highly sensitive and specific for disease detection, and much feasible and economic for test-in-field or onsite. This paper briefly describes the development and production of AIV H5 subtype-specific MAb to enhance MAb-based assays (e.g., Dot-ELISA and Biosensor) for the rapid detection of AIV
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
