Abstract
The aim of this work is to study of standardization parameters and pharmacological properties of Harpagophytum procumbens (H. procumbens) root dry extract.
 Materials and methods – obtaining of H. procumbens root dry extract was performed in accordance with developed scheme that is characterized by simplicity and cost-effectiveness of technology compared to analogous scheme. Study of standardization parameters of obtained extract was performed in accordance with the requirements of the monograph «Harpagophyti extractum siccum» of the State Pharmacopoeia of Ukraine 2.2., harmonized with the monograph «Harpagophyti extractum siccum» of the European Pharmacopoeia 9.5. Pharmacology study of analgesic and anti-inflammatory activities were conducted on the rat paw formalin-induced edema model.
 Results. Obtained H. procumbens root dry extract is a free flowing, non-hygroscopic, light brown powder with taste and odor that is specific to raw material of H. procumbens. Determined loss on drying was 3.2±0.18 %. Content of heavy metals in dry extract was not more than 0.01 % (100 ppm). Harpagoside was identified by TLC method. Chromatographic zones on the obtained chromatograms of the reference solution and test solutions are the same in intensity of a color, cross-over and sharpness of development. Based on the results of the research performed by HPLC method, it has been established that content of harpagoside in investigated H. procumbens root dry extract is 2.50±0.02 %.
 According to the results of pharmacological research it was found that investigated dry extract (the content of harpagoside is not less than 37.0 mg/kg) showed a higher activity compared to reference drug («Phong Te Thap»).
 Conclusions. A new method of obtaining dry extract of H. procumbens (3.5:1) was proposed. The developed green production technology is simple; it does not require special equipment and expensive solvents. A correspondence of quality parameters with the requirements of State Pharmacopoeia of Ukraine and European Pharmacopoeia: appearance, identification of harpagoside and fructose, loss of drying, content of heavy metals, assay of harpagoside (2.50±0.02 %) was set. Analgesic and anti-inflammatory activities of H. procumbens dry extract has been established
Highlights
In the entire world, in Ukraine, musculoskeletal system disorders occupy one of the leading places among the reasons of disability of population followed by accompanied premature loss of working efficiency
Every year losses of population-based labor work capacity are constantly increasing; this is a socially significant problem [1,2,3]. This group of diseases is characterized by inflammation of joints and chronic pain syndrome that requires long-term administration of synthetic medicines such as nonsteroidal anti-inflammatory drugs (NSAIDs)
Phytotherapy of musculoskeletal diseases is based on the selection of medicinal plant raw material, which have anti-inflammatory, diuretic, analgesic, sedative, health-promoting, desensitizing activity, in other words it has an effect on the etiological factors and clinical symptoms of diseases [6, 7]
Summary
In Ukraine, musculoskeletal system disorders occupy one of the leading places among the reasons of disability of population followed by accompanied premature loss of working efficiency. Every year losses of population-based labor work capacity are constantly increasing; this is a socially significant problem [1,2,3]. This group of diseases is characterized by inflammation of joints and chronic pain syndrome that requires long-term administration of synthetic medicines such as nonsteroidal anti-inflammatory drugs (NSAIDs). To avoid their negative effect on the patient’s body during remission, herbal medicines can be treatment of choice. The following plants are used in the international medical practice: Zingiber officinale, Сurcuma domestica, Harpagophytum procumbens, Scrophularia nodosa, Warburgia salutaris, Capsicum frutescens, Gaultheria procumbens [8,9,10]
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