Development of green, high throughput, and sensitive universal microwell spectrofluorimetric method for one-step determination of sartans in their dosage forms and plasma

Abstract

This study describes, for the first time, the development of a novel one-step universal and green microwell spectrofluorimetric method with high sensitivity and throughput for the determination of sartans. The method was developed using 4 sartans, as representative examples. These saratns were losartan potassium, irbesartan, candesartan cilexetil, and valsartan. The method comprised the measurement of enhanced native fluorescence of sartans in acidic solutions by using a fluorescence microplate reader at 260 and 410 nm for excitation and emission, respectively. The variables affecting the fluorescence intensity were optimized. The method was linear in a range of 20–1000 ng/mL. The limits of detection ranged between 7.3 and 13.4 ng/mL, and those of quantitation ranged between 22.5 and 40.6 ng/mL. The proposed method showed high levels of precision and accuracy. The method was successfully applied to the estimation of sartans in their dosage form, in-vitro drug release testing, and analysis of plasma samples. The proposed method is the first spectrofluorimetric method that can analyze all sartans on a single analysis system without alterations in the measuring wavelengths. The greenness of the method was assessed using three distinct metric tools, which conclusively demonstrated its adherence to green analytical principles. Moreover, the method has high throughput capacity as it is able to concurrently process large number of micro-volume samples. In conclusion, the proposed method is valuable for the rapid and routine determination of sartans for the purposes of quality control and bioequivalence studies.