Abstract
Tebufenozide is a non-steroidal insect growth regulator, which has been widely used in agriculture to control pests. Despite being recognized as an eco-friendly pesticide, its residues still can pose potential health hazards to humans. In this study, two haptens of tebufenozide were synthesized to prepare the monoclonal antibodies (mAbs). Out of the eight mAbs, mAb 5C5B6 exhibited the highest sensitivity (half maximal inhibitory concentration = 0.15 ng/mL) in the heterologous indirect competitive enzyme-linked immunosorbent assay, and was utilized to develop gold nanoparticle lateral flow immunoassay (LFIA). The cut-off value and visual limit of detection of the LFIA were 10 and 1.25 ng/mL. The detection results of both the spiked and blind samples demonstrated that the proposed LFIA exhibits high accuracy in detecting tebufenozide in agricultural products.
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