Abstract
The assessment of genetic structure and genetic diversity is crucial for management and conservation. A low number of markers may fail to identify robust population structure. A solution of this shortcoming can be high-throughput sequencing that allows genotyping thousands of markers on a genome-wide approach. We used Genotyping-by-sequencing (GBS) approach on 60 individuals of Sebastiscus marmoratus, from three locations in Chinese coastal waters. We discovered a total of 7376 genome-wide single nucleotide polymorphisms (SNPs). After applying careful filtering criteria, we obtained 298 high-differentiated SNPs that performed well for population genetics and population assignment. The 298 SNPs provided high power for population assignment with only one misassignment among three populations. SNP validation confirmed the existence and the high quality of this filtered SNPs. The SNPs in our study could be used as powerful markers in further genetic and evolutionary studies.
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