Abstract
In this study, we focused on the DSB repair machinery as a mechanism of asexual pathogenic and genomic evolution in P. oryzae. Using the fungal TALEN system, we demonstrated that DSBs trigger genome rearrangement with deletions, duplications, and translocations of AVR-Pita through somatic and octopic HR between repetitive sequences in the genome. In addition, based on the unique DSB repair machinery of P. oryzae, we developed original genome editing technologies using the fungal CRISPR/Cas9 system. These developed genome editing technologies would accelerate high-throughput functional genomics and reveal genome evolution mechanisms in plant pathogenic fungi.
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