Abstract
The genes coding for toluene monooxygenase (TMO), an enzyme also capable of degrading trichloroethylene (TCE), from Pseudomonas mendocina KR1 were cloned into both narrow‐ and broad‐host‐range vectors and introduced into Escherichia coli and P. putida. TMO activity expressed by a number of strain/plasmid combinations was assayed by determining decreases in the fluorescence of indble. Degradation of TCE was investigated in sealed serum vials for a number of these combinations. Finally a kinetic experiment, involving determination of the rate of TCE degradation at various TCE concentrations, was performed, and approximate kinetic constants for TCE degradation by these two species were determined.
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