Development of Gelatin Methacryloyl Hydrogel loaded ZnS Nanoparticles Patches for In vivo wound healing care, In vitro drug release and free radical scavenging evaluations

Abstract

An open wound is significantly vulnerable to polymicrobial infections leading to higher level of inflammatory responses. For rapid healing processes, a wound greatly needs a biologically compatible wound dressing with unique properties including hydrophilicity, mechanical strength, anti-microbial, anti-oxidant abilities. In this present research investigation, biodegradable gelatine methacryloyl (GelMH) hydrogel loaded with zinc sulphide nanoparticles (ZnS NPs) was developed for improving the wound healing efficiency. The synthesized materials were carefully characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Scanning Electron Microscopy to confirm formation of ZnS NPs. Then it was tested for swelling, degradation, In vitro drug release, DPPH Free Radical Scavenging and in vivo wound care potential. The free radical scavenging assays clearly revealed that the GelMH loaded ZnS NPs patches could efficiently inactivate experimentally produced free radicals. In vitro drug release also reveals that the GelMH loaded ZnS NPs patches is constantly released on PBS for at least 15 days until incubation. The wound healing study indicated that GelMH loaded ZnS NPs patches could effectively promote the wound healing. From the overall analysis, it is observed that GelMH loaded ZnS NPs patches is highly excellent promising materials for the therapeutic potential of wound care management.